中英文介绍丨艾美捷NEUROMAG 200 磁转染试剂

NeuroMag Transfection Reagent is the first dedicated Magnetofection™ transfection reagent for neurons from 1 DIV to 21 DIV. It has proven to be extremely efficient in transfecting a large variety of primary neurons such as cortical, hippocampal, dorsal root ganglion and motor neurons with all types of nucleic acids. Moreover, high transfection efficiency was also achieved in primary astrocytes, oligodendrocyte precursors or neural stem cells as well as other cell lines (C6, B65, PC12, N2A...). Due to its unique properties, NeuroMag allows following transfected neurons during several days.

Great efficiency, ideal for primary neurons

Efficient from 0 DIV to 21 DIV

Non toxic and completely biodegradable: high transfected neurons viability

Ready-to-use, straightforward and rapid

For all types of nucleic acids

艾美捷NEUROMAG 200 磁转染试剂#NM50200是一款专用试剂磁感应™用于 1 DIV 至 21 DIV 神经元的转染试剂。事实证明，它在用所有类型的核酸转染各种原代神经元（如皮质、海马、背根神经节和运动神经元）方面非常有效。此外，在原代星形胶质细胞、少突胶质细胞前体或神经干细胞以及其他细胞系（C6、B65、PC12、N2A等）中也实现了高转染效率。由于其独-特的性质，NeuroMag允许在几天内跟踪转染的神经元。

NEUROMAG 200 磁转染试剂特点：

效率高，是原代神经元的理想选择

从 0 DIV 到 21 DIV 的高效

无毒且完-全可生物降解：高转染神经元活力

即用型、简单、快速

适用于所有类型的核酸

NEUROMAG 200 磁转染试剂结果示例：

图1：用 NeuroMag 有效转染的大鼠原代海马神经元。在1孔板中转染3 μg pVectOZ-GFP和5.24 μL NeuroMag的大鼠原代海马神经元的代表性图像。转染后48h荧光下拍摄照片

图2：表达GFP的小鼠皮质神经元（培养3周，磁转染后2-3天）。图像采集：配备A1激光扫描共聚焦系统和60倍Apo TIRF物体镜头的尼康Ti-E显微镜。结果由C. Charrier博士提供（Charrier C.等人，2012年，Cell，第149卷，第4期，923-935）。