中英文介绍丨艾美捷IN VIVO POLYMAG 500 转染试剂

In vivo PolyMag™ Transfection Reagent has been designed for in vivo targeted transfection of various types of nucleic acids such as DNA, RNA, oligonucleotides using magnetic nanoparticles coated with specific polymers.  

DNA complexes can be easily administrated through various injection routes such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

In vivo Magnetofection™ main advantages in comparison to other procedures:

Increased transfection efficiency

Targeted process (magnetically-driven)

Reduction of the systemic dissemination of vectors during injection

Reduction of the vector doses

Work under non permissive conditions (hypothermia, physiological flow conditions)

Penetration of the vector into tissues

Minimized toxicity

Universal – suitable for all nucleic acids

艾美捷IN VIVO POLYMAG 500 转染试剂#IV-PN30500设计用于使用涂有特定聚合物的磁性纳米颗粒对各种类型的核酸（如DNA、RNA、寡核苷酸）进行体内靶向转染。

DNA复合物可以通过各种注射途径轻松给药，例如全身给药（静脉内，动脉内）或局部给药（肿瘤内，脑室内）。

IN VIVO POLYMAG 500 转染试剂的结果示例：

图1：IN VIVO PolyMag 在静脉注射后靶向基因递送的高效率。荧光素酶表达42h后将与IN VIVO PolyMag纳米颗粒复合的报告基因注射到兔耳动脉中。基因表达主要在磁体位置位点发现。作为对照，在不施用磁铁的情况下将相同的载体组合物注入对侧血管中。未发现显著的报告基因表达。

图2：在高度严格的条件下，IN VIVO PolyMag 在难以转染的组织中递送 DNA 的高效率。将DNA复合物和体内PolyMag纳米颗粒注射到不存在（A）或在磁场（B）影响下的大鼠的回肠中。48小时后进行X-Gal染色，在宏观水平（上图）和微观水平（下图）存在磁铁（B）的情况下，揭示了高效的基因递送。在没有磁场的情况下，仅观察到罕见的转染事件（A）