艾美捷Calcofluor白色染色剂的属性及相关研究方案

Calcofluor white dye is a fluorescent blue dye that can bind to cellulose and chitin, which are present in the cell walls of fungi, algae, and plants. In budding yeast, the Calcofluor white stain stains the bud scars more strongly because they contain higher concentrations of chitin. Calcofluor white staining is sometimes used to identify fungal infections in medical samples.

Calcofluor白色染色剂是一种荧光蓝色染料，可与纤维素和几丁质结合，后者存在于真菌、藻类和植物的细胞壁中。在出芽酵母中，Calcofluor 白色染色剂对芽痕的染色更强烈，因为它们含有更高浓度的几丁质。Calcofluor 白色染色剂 有时用于识别医学样本中的真菌感染。

液体培养细胞染色方案：

该染色方案使用酿酒酵母进行了优化在纯粹的文化中。可能需要确定合适染料浓度对其他生物体进行实验。

1.在适当的生长培养基中培养细胞。可选：降速和再悬浮在适合成像的透明缓冲液中，例如PBS或HBSS。

2.将染料以最终浓度直接添加到生长培养基或缓冲液中的细胞中为5-25μM。

3.在室温或30°C下培养20分钟，摇动，防止光可选：成像前清洗一次。

4. 使用DAPI发射滤光片在荧光显微镜上成像。

艾美捷Calcofluor白色染色剂#29067属性：

激发/发射：360/430 纳米

分子量：916.98

储存条件：储存在-10至-35°C，避光

A） 用25μM Calcofluor White染色的酿酒酵母20分钟，然后使用蔡司LSM700共焦显微镜成像DAPI成像设置。B） 酿酒酵母顺序染色用1μM ViaVac红/绿和25μM Calcofluor白在PBS中20分钟。使用DAPI和Texas Red®在蔡司LSM700共焦显微镜上成像设置。

Calcofluor白色染色剂相关研究：

Catalog number      Product   Function

29068      ViaVac  Red/Green,  10  mM  in  DMSO   Yeast vital dye

40077      Thiazole  Orange,  10 mM in  DMSO   Live cell fixable cytoplasmic stain

31062      Yeast Vitality Staining Kit   Calcofluor White to stain the cell wall, ViaVac Red/Green as a vital dye

31063      Yeast Viability Staining Kit   ConA to stain cell walls, Live-or-Dye to stain dead cells

31064      Yeast Fixable Live/Dead Staining Kit   Thiazole Orange to stain all cells, Live-or-Dye to stain dead cells

29015-29020;29058      CFTM   Dye   ConcanavalinA(Con   A)   Cell wall stain in a variety of dye conjugates

29021-29029; 29059;29064      CFM  Dye  Wheat  Germ  Agglutinin  (WGA)   Cell wall bud scar stain in a variety of dye conjugates

32002-32009      Live-or-Dye Fixable Viability Staining Kits   Dead-cell-specific viability stains in a variety of dye colors

23001      EverBrite  TM Mounting  Medium   Wet  set mounting medium for microscopy

23002      EverBrite M Mounting Medium with DAPI   Wet set mounting medium for microscopy containing DAPI