中英文说明丨艾美捷正常山羊血清#005-000-121方案

Normal serum is used to describe the serum from animals that have not been immunized with specific antigens to produce antibodies. Therefore, normal serum is opposite to antiserum. Normal serum contains general complement of serum proteins, including different kinds of immunoglobulins that exist in healthy animals of specific species. The normal serum products provided are extracted with lipids to improve transparency, and desalted and dialyzed to remove phosphate buffer containing sodium azide, And finally freeze-dried to obtain freeze-dried powder. Normal serum is very suitable as a blocking reagent to remove non-specific hybridization. Normal serum is often used as a control when detecting general or specific antibody purification methods.

Amyjet normal goat serum # 005-000-121, which comes from a non immune goat host, is purified by lipid extraction and dialysis with PBS solution containing sodium azide to improve its transparency. It is recommended to use PBS to dilute the sealed serum stock solution 5-20 times, prepare it into a working solution of 5% -20%, and directly drip it onto tissue slices or cell smears. Incubate at 37 ℃ for 10 to 30 minutes, remove the serum, do not wash it, and drip an appropriate proportion of diluted primary antibody working solution. Incubate at 37 ℃ for 1-2 hours or 4 ℃ overnight. Note: The serum used for blocking cannot contain the target protein and is different from the source of the first antibody. Blocking serum from the same source as the second antibody can be used.

正常血清是用来描述来自没有用特异抗原进行免疫产生抗体的动物的血清.因此，正常血清与抗血清相对.正常血清包含血清蛋白的一般补体，包括存在于特定物种健康动物体内的不同种类的免疫球蛋白.提供的正常血清产品经过脂类抽提以提高透明度，经去盐和透析处理以去除包含叠-氮-化-钠的磷酸缓冲液，并最后冻干以获得冻干粉末.正常血清非常适用于作为封闭试剂去除非特异性杂交.正常血清在检测一般或特异抗体纯化方法的时候也经常作为对照使用.

艾美捷正常山羊血清#005-000-121，来源于非免疫的山羊宿主，经过脂质萃取和含叠-氮-化-钠的PBS溶液透析，改善其透明度。推荐用PBS将封闭血清原液稀释5-20倍，配成5%-20%的工作液，直接滴加在组织切片或者细胞涂片上，37℃度孵育10-30分钟，清除血清，勿洗，滴加适当比例稀释的一抗工作液，37℃孵育1-2h或4℃过夜。注意：封闭用血清中不能含有目标蛋白，且与一抗来源不同，可用与二抗相同来源的封闭血清。

正常山羊血清#005-000-121基本参数：

中文名称：正常山羊血清

英文名字：Normal Goat Serum

编号：005-000-121

规格：10ml

物理状态：冻干固体

建议工作浓度或稀释范围：

出于封闭目的，使用5%（v/v）溶液（从再水合体积中稀释1:20）。按照说明重新补水可产生100%的血清。

描述：该正常山羊血清产品可应用于大多数免疫学实验，用作封闭液或空白对照.

保存建议：冻干粉保存在2-8°C，准备用时，根据指示的体积加入双蒸水，在2-8°C条件下，未稀释的液体保质期6周，每天准备新鲜的工作液.如果重溶后，想延长保存期，将未稀释的溶液保存在-20°C或者更低，避免反复冻融.