中英文介绍丨艾美捷Murashige & Skoog培养基含维生素

MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium and originally developed for the c*tion of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.

Micro Elements mg/l µM

CoCl2.6H2O 0.025 0.11

CuSO4.5H2O 0.025 0.10

FeNaEDTA 36.70 100.00

H3BO3 6.20 100.27

KI 0.83 5.00

MnSO4.H2O 16.90 100.00

Na2MoO4.2H2O 0.25 1.03

ZnSO4.7H2O 8.60 29.91

Macro Elements mg/l mM

CaCl2 332.02 2.99

KH2PO4 170.00 1.25

KNO3 1900.00 18.79

MgSO4 180.54 1.50

NH4NO3 1650.00 20.61

Vitamins mg/l µM

Glycine 2.00 26.64

myo-Inositol 100.00 554.94

Nicotinic acid 0.50 4.06

Pyridoxine HCl 0.50 2.43

Thiamine HCl 0.10 0.30

Total concentration Micro and Macro elements including vitamins: 4405.19 mg/l

Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Murashige & Skoog培养基含维生素背景：

MS（Murashige & Skoog）培养基是极为常用的组织培养基，已经开发出许多改良形式。该培养基起源于White培养基，最初是为了种植烟草的愈伤组织而开发的。与White培养基相比，所有成分的浓度都增加了。增加到50-60 mM的氮会明显刺激烟草细胞的生长，但是80 mM和更高的浓度显然对细胞不利。所有其它元素（尤其是大量元素）的增加也刺激了愈伤组织的生长。由于矿物质的浓度很高，MS培养基是非常丰富和含盐量高的培养基，对于某些植物物种可能太“咸”。为避免此问题，MS培养基通常与全浓度的微量元素一起使用，而大量元素的浓度分别为浓度的一半或四分之三。有时，原来的MS培养基种维生素被Linsmaier和Gamborg B5培养基中的维生素所取代，因为植物对硫胺素的需求量较大。Duchefa拥有多种MS 培养基方案。

Murashige & Skoog培养基含维生素#M0222.0050组成：

微量元素 毫克/升 微米

氯化钴2.6H2O 0.025 0.11

铜酸4.5H2O 0.025 0.10

菲纳多达 36.70 100.00

H3博3 6.20 100.27

基 0.83 5.00

锰硫4.H2O 16.90 100.00

那2哞4.2H2O 0.25 1.03

氧化锌4.7H2O 8.60 29.91

宏元素 毫克/升 毫米

氯化钙2 332.02 2.99

韩2采购订单4 170.00 1.25

诺3 1900.00 18.79

镁硫磺4 180.54 1.50

新罕布-什尔州4不3 1650.00 20.61

维生素 毫克/升 微米

甘-氨-酸 2.00 26.64

肌醇肌醇 100.00 554.94

烟-酸 0.50 4.06

盐酸吡哆醇 0.50 2.43

盐酸硫胺素 0.10 0.30

包括维生素在内的微观和宏观元素总浓度：4405.19毫克/升

Murashige T.和Skoog F.，Physiol. Plant，15，473（1962）。