艾美捷小鼠TNF-α ELISA试剂盒参数和操作步骤分析

Mouse TNF- αThe ELISA kit Detection principle：

Mouse TNF- αThe ELISA kit uses a double antibody sandwich ELISA method. Using anti mouse TNF- α Antibodies are coated on enzyme labeled plates, and the mouse TNF in the sample or standard during the experiment- α Binding to the coated antibody, the free components are washed away. Add biotinylation anti mouse TNF in turn- α Antibodies and horseradish peroxidase labeled avidins. Anti mouse TNF- α Antibodies and Mouse TNF Binding to Coated Antibodies- α Binding, biotin and avidin specifically bind to form immune complexes, and free components are washed away. By adding a chromogenic substrate (TMB), the TMB appears blue under the catalysis of horseradish peroxidase, and turns yellow after adding a termination solution. Using an enzyme-linked immunosorbent assay (ELISA) to measure the OD value at a wavelength of 450nm, mouse TNF- α The concentration is proportional to the OD450 value, and the mouse TNF in the sample is calculated by plotting a standard curve- α Concentration of.

艾美捷小鼠TNF-α ELISA试剂盒#NDC-KSP-ZWL3G1-96采用定量酶免疫测定技术，测量样品中的特定抗原。

小鼠TNF-α ELISA试剂盒相关组成：

小鼠TNF-α ELISA试剂盒基本参数：

应用 酶联免疫吸附法

描述 小鼠 TNF-α 酶联免疫酶抑制剂试剂盒

类型 检测与检测

范围 7.8-500 皮克/毫升

研究领域 细胞信号传导

样品类型 血清，血浆

敏感性 1.0 皮克/毫升

规格 96t

物种反应性 鼠

小鼠TNF-α ELISA试剂盒操作步骤：

1. 在各孔中加入标准品或样品各100μL, 37℃孵育90分钟

2. 加入100μL 生-物-素化抗体工作液,37℃孵育60分钟

3. 洗涤3次

4. 加入100μL酶结合物工作液, 37℃孵育30分钟

5. 洗涤5次

6. 加入90μL底物溶液, 37℃孵育15分钟左右

7. 加入50μL终止液,立即在450nm波长处测量OD值

8. 结果计算