中英文说明丨艾美捷ATTO488-葡聚糖

Synthesis and structure

Atto™-dextrans are synthesised from well-characterized dextran fractions derived from Leuconostoc mesenteroides via the succinimidyl-derivative of Atto488. After purification from non-bound dye, the products are controlled for Mw, solubility, DS, free dye and loss on drying.

The products are designated by the approximate molecular weights of the dextran fractions used. Thus, for example, the product Atto-dextran 4 has a molecular weight of approx. 4000. The actual molecular weight is determined by GPC. This value is supplied with the Certificate of Analysis. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear α-(1-6)-linked

glucose chain with however a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran fractions used are from Mw of 4000 to 150000 and are carefully controlled by GPC, optical rotation, absorbance and other control parameters.

ATTO488-葡聚糖的结构特征

Spectral properties

Atto488-dextran has an absorbance maximum at 502 nm (in borate buffer, pH 9.0) and an emission wavelength of 524 nm. The Atto-dye has a somewhat higher quantum yield than fluorescein and displays a better photo-stability. The DS lies between 0.001-0.008.

Applications

Atto488-dextrans are primarily used for studying permeability and transport in cells and tissues. An added bene¬fit is that measurements of the fluorescence provide quantitative data on the permeability of healthy and diseased tissues. Such studies can be performed in real time by intravital fluorescence microscopy. The technique offers high sensitivity and concentrations down to 1μg/ml can be detected in tissue fluids

艾美捷ATTO488-葡聚糖#AT488D4/AT488LD10, 以橙色粉末形式提供，易溶于水。ATTO488-葡聚糖主要用于研究细胞和组织中的渗透性和转运。

ATTO488-葡聚糖是由来源于明串珠菌的具有良好特征的右旋糖聚糖组分合成的通过Atto488的琥珀酰亚胺衍生物形成的肠系膜。从非结合染料中纯化后控制产品的Mw、溶解度、DS、游离染料和干燥损失。产品由所用葡聚糖部分的近似分子量指-定。因此，例如，产物Atto-右旋糖酐4具有约4000的分子量。实际分子量通过GPC测定。此值随分析证书一起提供。这个所用的葡聚糖来自间质明串珠菌B-512F，其本质上是线性α-（1-6）连接的然而，沿链分布的α-（1-3）分支的百分比较低（2-5%）。所使用的葡聚糖级分的Mw为4000至150000，并通过GPC、光学转速、吸光度和其他控制参数。

物理特性：

Atto-葡聚糖是一种橙色粉末，可自由溶解于水或电解质溶液中。这个由于存在磺酸盐和氨基部分。Atto-葡聚糖不溶于大多数有机溶剂，丙酮、氯仿、乙酸乙酯等。

应用：

Atto488葡聚糖主要用于研究细胞和组织中的渗透性和转运。一额外的好处是荧光测量提供了关于健康和患病组织的渗透性。这种研究可以通过活体内注射实时进行荧光显微镜。该技术具有高灵敏度，浓度低至1μg/ml可以在组织液中检测到。

相关研究：

AT488LD10 ATTO488-lysine-dextran 10 10000 5mg

AT647NLD70 ATTO647N-lysine-dextran 70000 5mg