中英文说明丨艾美捷木葡聚糖（克隆LM25）

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall.

Product Information：

Immunogen Neoglycoprotein, Xyloglucan

Host Rat

Clonality Monoclonal

Subclass/isotype IgM

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term).

Tested applications ELISA (ELISA), Immuniflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

艾美捷木葡聚糖（克隆LM25）#AS18-4205-1ml详细信息：

免疫原：新糖蛋白，木葡糖聚糖

宿主：大鼠

单克隆：克隆

亚类/同种型：IgM

纯度：细胞培养上清液。

形态：液体

数量：1毫升

储存温度：为+4°C（短期）和-20°C（长期）。

测试应用：ELISA（ELISA）、免疫荧光（IF）

推荐稀释度：1:10（ELISA，IF）

已确认的反应性：高等植物、蕨类植物和苔藓

无反应性：目前还不知道预测反应性的已确认例外情况

Agrisera木葡聚糖（克隆LM25）应用示例：

Material: Cassava leaf, epidermal layer

Fixation: aldehyde, embedded in HM20 resin, thin sectioned

Blocking: 4% milk in PBS

Primary antibody: Anti-xyloglucan, LM25 (AS18 4205-1ml)

Secondary antibody (Agrisera): Donkey anti-Rat IgG (H&L), DyLight® 594 conjugated, AS12 2090 (red)

Counterstained with Calcofluor White (blue). Super-resolution images were acquired on a ZEISS Elyra7 in Lattice Structured Illumination Microscopy mode.

Courtesy Dr. Kirk Czymmek, Donald Danforth Plant Science Center, USA

Agrisera木葡聚糖（克隆LM25）文献参考：

Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.